Annotate a Phosphoproteomic Dataset

Upload a CSV or TSV file, identify the UniProt accession and peptide columns, choose annotation options, and download the result enriched with gene names, domains, GO terms, and site-level information from ProteomeScout.

1Upload Dataset

Select file (CSV or TSV):

Maximum file size: 100 MB. The file must contain a header row with column names.

2Select Columns

UniProt/SwissProt Accession Column

Peptide Sequence Column

Peptides must have modified residues written in lowercase (e.g. ANsFVGTAQYV for phospho-S241). All other residues should be uppercase.

3Annotation Options

Find modification sites – locates each modified residue within the full protein sequence and adds position, domain, macro-molecular structure, and secondary structure context

Include GO terms – appends Gene Ontology terms (Molecular Function, Biological Process, Cellular Component) to each row

4Annotate & Download

Annotation queries ProteomeScout for every row in your dataset. Processing time scales with dataset size — a few hundred rows typically completes in under a minute.